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M-CSF, Mouse-Z03275

2025-07-18

產(chǎn)      地:
暫無
所在地區(qū):
福建廈門市
有效期還剩 360舉報該信息

Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain, and it is only active in the disulfide-linked dimeric form, which is bonded at Cys63.


產(chǎn)品簡介

Species: Mouse

Protein Construction :Expressed with an N-terminal Met. M-CSF (Lys33-Pro187) Accession # P07141

Purity :> 95% as analyzed by SDS-PAGE

Endotoxin Level :< 0.2 EU/μg of protein by gel clotting method

Biological Activity: ED50 < 3.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 3.3 × 105 units/mg.

Expression System: E. coli

Apparent Molecular Weight :~30 kDa, on SDS-PAGE under non-reducing conditions.

Formulation: Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.

Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml. Storage & Stability: Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.


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