活性氧（ROS）引起的脂質氧化損傷在一些疾病、細胞功能障礙和衰老過程中起著重要作用。丙二醛（MDA）和4-羥基-2-壬烯醛（4-HNE）等醛類被認為是脂質過氧化的晚期產(chǎn)物。然而，最近在脂質過氧化的早期階段，發(fā)現(xiàn)13-羥基過氧化十八碳烯酸（13-HPODE）可以與蛋白質共價結合。己酰賴氨酸加合物（HEL）是一種新型的脂質過氧化氫修飾的賴氨酸殘基。HEL是由亞油酸或花生四烯酸等ω-6脂肪酸的氧化修飾形成的。這種單克隆抗體（克隆5H4）特異性針對含有HEL的蛋白質和肽段。適用于通過免疫組化和西方印跡法檢測HEL。

艾美捷JaICA抗己酰賴氨酸（HEL）單克隆抗體：

貨號：MHL-021P

克隆號： 5F12

亞類： IgG1 kappa

抗原： N-ε-己酰-鑰孔血藍蛋白（HEL-KLH）

特異性： 特異性針對己酰賴氨酸加合物。

形式： 凍干粉。含有10 mM PBS（pH 7.4）、5%蔗糖、1%牛血清白蛋白和0.05% Procline 950。IgG濃度為100微克/毫升。

應用： 免疫組化（建議在甲醛固定的組織上使用2微克/毫升的抗體濃度）和ELISA

儲存： -20°C以下保存

左圖：用順鉑處理的大鼠腎臟。（Sugiyama博士，鳥取大學）

右圖：在人類動脈粥樣硬化病變中檢測HEL。（Naito博士）

文獻參考：

1)Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)

Identification of HEL, which is lysine adduct of 13-HPODE.

2)Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)

Development and characterization of anti HEL monoclonal antibody.

3)Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)

Application to rabbit atherosclerotic lesions.

4)Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)

Application to cultured cells (HUVECs).

5)Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)

Application to cultured cell from mouse.