Cy5 酰肼 Cyanine5 hydrazide

C32H42Cl2N4O

569.61

5mg

25mg

dark blue powder

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

-20°C

Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.

This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.

Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649 dyes.

Spectral properties

Product citations

1. Boone, C.; Grove, R.; Adamcova, D.; Braga, C.; Adamec, J. Revealing oxidative damage to enzymes of carbohydrate metabolism in yeast: An integration of 2D DIGE, quantitative proteomics and bioinformatics.Proteomics, 2016, 16(13), 1889–1903. doi: 10.1002/pmic.201500546
2. Yoshimura, T.; Harashima, M.; Kurogi, K.; Suiko, M.; Liu, M.-C.; Sakakibara, Y. A novel procedure for the assessment of the antioxidant capacity of food components. Analytical Biochemistry, 2016, 507, 7–12. doi:10.1016/j.ab.2016.05.002
3. Huang, J.; Smith, F.; Panizzi, J.R.; Goodwin, D.C.; Panizzi, P. Inactivation of myeloperoxidase by benzoic acid hydrazide. Archives of Biochemistry and Biophysics, 2015, 570, 14–22. doi:10.1016/j.abb.2015.01.028
4. Einfalt, T.; Goers, R.; Dinu, I.A.; Najer, A.; Spulber, M.; Onaca-Fischer, O.; Palivan, C.G. Stimuli-triggered activity of nanoreactors by biomimetic engineering polymer membranes. Nano Letters, 2015, 15(11), 7596–7603. doi: 10.1021/acs.nanolett.5b03386