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*/ 該細(xì)胞為ATCC源細(xì)胞，由試劑銷售引進(jìn) /*
FHs 74 Int細(xì)胞人小腸上皮細(xì)胞?
ATCCâ Number: CCL-241™
Lot Number: 70019552
Name: FHs 74 Int
Description: Small Intestine
Species: Human (Homo sapiens)
Volume/Ampule: Approximately 1 mL
Date Frozen: 09JAN2019
Recovery: A T-25 setup at a seeding density of 1.5 x 104 viable cells/cm2 reaches approximately 15% confluence in 1 day and 90% confluence in 3 days.
Product Format: Cells cryopreserved in the appropriate cryopreservation medium Expiration Date:Not applicable
Storage Conditions: Vapor phase of liquid nitrogen
FHs 74 Int細(xì)胞人小腸上皮細(xì)胞?
Test / Method | Specification | Result |
Ampule passage number | Report results | 22 |
Population doubling level (PDL) | Report results | Not applicable |
Total cells/ampule (Cell count using Trypan Blue stain method) | Report results | 4.4 x 105 total cells/ampule |
Post-freeze viability (Cell count using Trypan Blue stain method) | ≥ 50.0% | 90.8% |
Growth properties (Visual observation method) | Adherent | Adherent |
Morphology (Visual observation method) | Epithelial-like* | Epithelial-like |
Test for mycoplasma contamination Hoechst DNA stain (indirect) method Agar culture (direct) method PCR-based assay |
None detected None detected None detected |
None detected None detected None detected |
Species determination: COI assay (interspecies) | Human | Human |
ATCCâ Number: CCL-241™
Lot Number: 70019552
Species determination: STR analysis (intraspecies) | Human (Unique DNA Profile) TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11, 12 D7S820: 9, 10 D16S539: 11, 12 CSF1PO: 10, 11 Amelogenin: X vWA: 16, 18 TPOX: 8 | Human (Unique DNA Profile) TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11, 12 D7S820: 9, 10 D16S539: 11, 12 CSF1PO: 10, 11 Amelogenin: X vWA: 16, 18 TPOX: 8 |
Sterility test (BacT/ALERT 3D) iAST bottle (aerobic) at 32.5°C iNST bottle (anaerobic) at 32.5°C |
No growth No growth |
No growth No growth |
Human pathogenic virus testing (PCR-based assay for HIV, HepB, HPV, EBV, and CMV) | Report results | HIV – None detected HepB – None detected HPV – None detected EBV – None detected CMV – None detected |
* Epithelial-like: Any adherent cells of a polygonal shape with clear, sharp boundaries between them.
Quality Assurance Specialist; Quality Assurance
ATCC hereby represents and warrants that the material provided under this certificate is pure and has been subjected to the tests and procedures specified and that the results described, along with any other data provided in this certificate, are true and correct to the best of the company’s knowledge and belief. This certificate does not extend to the growth and/or passage of any living organism or cell line beyond what is supplied within the container received from ATCC.
This product is intended to be used for laboratory research use only. It is not intended for use in humans, animals, or for diagnostics. Appropriate Biosafety Level (BSL) practices should always be used with this material. Refer to the Product Information Sheet for instructions on the correct use of this product.
ATCC products may not be resold, modified for resale, used to provide commercial services, or to manufacture commercial products without prior written agreement from ATCC.
© 2017 ATCC. The ATCC trademark and trade name are owned by the American Type Culture Collection.
FHs 74 Int細(xì)胞人小腸上皮細(xì)胞?
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