Rabbit Tumor necrosis factor α(TNF-α) ELISA Kit
兔腫瘤壞死因子α(TNFα)ELISA試劑盒實驗原理 ：
本試劑盒用于測定兔血清，血漿及相關液體樣本中腫瘤壞死因子α（TNF-α）的含量。應用雙抗體夾心法測定標本中兔腫瘤壞死因子α（TNF-α）水平。用純化的兔腫瘤壞死因子α（TNF-α）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入腫瘤壞死因子α，再與 HRP 標記的羊抗兔抗體結合，形成抗體-抗原-酶標抗體復合物，經(jīng)過*洗滌后加底物 TMB 顯色。TMB 在 HRP 酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的兔腫瘤壞死因子α呈正相關。用酶標儀在 450nm 波長下測定吸光度（OD 值），通過標準曲線計算樣品中兔腫瘤壞死因子α（TNF-α）濃度。
Principle of the assay:
This kit allows for the determination of TNF-α concentrations in Rabbit serum, blood plasma, and other biological fluids.The kit assay Rabbit TNF-α level in the sample，use Purified Rabbit TNF-α antibody to
coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined antibody which With HRP labeled goat anti-rabbit become antibody - antigen -enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.

試劑盒供應商：上海華壹生物科技有限公司