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| 產(chǎn)品英文名稱 | IPKine™ Anti-HA Magnetic IP Kit |
| 產(chǎn)品中文名稱 | IPKine™ HA標(biāo)簽蛋白免疫沉淀試劑盒（磁珠法） |
| 免疫原 | 合成多肽 |
| 試劑盒組分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-HA Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer HA Peptide (25×) SDS-PAGE Loading Buffer (5×) |
| 特點(diǎn)&優(yōu)勢(shì) | ? 高效：特異性強(qiáng)、靶蛋白結(jié)合量高，≥0.6 mg HA標(biāo)簽融合蛋白/mL磁珠； ? 便捷：可結(jié)合多種形式的HA標(biāo)簽蛋白（N端HA融合蛋白、C端HA融合蛋白）； ? 通用：提供IP實(shí)驗(yàn)所需的所有必要緩沖液； ? 可靠：提供陰性對(duì)照，可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結(jié)合； ? 靈活：本試劑盒提供三種洗脫方法（HA多肽競(jìng)爭(zhēng)洗脫、酸洗脫和SDS-PAGE Loading Buffer洗脫方法）。 |
| 保存建議 | 按各組分標(biāo)簽提示分開(kāi)存儲(chǔ)，保質(zhì)期12個(gè)月。 |
| 運(yùn)輸條件 | 冰袋運(yùn)輸（藍(lán)冰） |
| 警告 | 本文列出的產(chǎn)品僅供研究使用，不適用于人類(lèi)或臨床診斷。我們產(chǎn)品所推薦應(yīng)用，不是建議使用我們的產(chǎn)品去違反任何或許可證。對(duì)于使用本產(chǎn)品可能發(fā)生的侵權(quán)或其他違規(guī)行為，我們不承擔(dān)任何責(zé)任。 |
| 背景 | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Figure. The immunoprecipitation effect of Anti-HA Magnetic IP Kit used for HA-Tag fusion protein. HEK293T cells were transfected with HA-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Elution Buffer; Lane 5 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Working HA Peptide. By using peptide elution and acid elution, only contained HA-Tag fusion protein, did not contain heavy and light chains of antibody.
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