99超碰中文字幕在线观看-天天干天天日天天舔婷婷-我看操逼的好看的女人的-日本一二三四五区日韩精品

武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells

時間:2021-11-11 閱讀:264
分享:

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells                            

1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from human main pulmonary artery.
2.A segment of the main pulmonary artery was excised and placed in a sterile 10-cm dish containing primary cell culture media.
3.The segment was stripped of adventitia with a sterile forceps.
4.The main pulmonary artery segment was then cut longitudinally to open the vessel, and the endothelial layer was removed by gentle rubbing with a cell scraper.
5.The vessel was then cut into 2-mm segments, inverted, and placed on a collagen-coated 35-mm tissue culture dish.
6.A drop of primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics was then added, and the cells were grown overnight at 37°C in a humidified atmosphere with 5% CO2-95% air.
7.The next day, an additional 2 ml of complete medium were added.
8.The growth medium was subsequently changed every 2 days.
9.When SMC islands could be observed under the microscope, the tissue segment was removed and the individual cell islands were subcloned.
10.Identity was confirmed as PASMCs by immunostaining (>99% positive) with SMC actin. This was taken as evidence that cultures were not contaminated with fibroblasts or endothelial cells.
11.All cultures for subsequent experiments were maintained in primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics at 37°C in a humidified atmosphere with 5% CO2-95% air.

References
1.Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture, Physiol. Res. 59, 1, 1979.
2.Richardson, M., Taylor, D., Casey, M., MacDonald, P., and Stull, J.: Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture, In Vitro Cell. Dev. Biol. 23, 21, 1987.
3.Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~
撥打電話 產(chǎn)品分類
在線留言
栾城县| 高邮市| 泰兴市| 乐安县| 永川市| 岚皋县| 石渠县| 五莲县| 伊春市| 博乐市| 成武县| 义乌市| 剑阁县| 麻栗坡县| 喀喇沁旗| 抚州市| 沅陵县| 临夏县| 浦东新区| 汝城县| 贡嘎县| 安化县| 名山县| 康定县| 安宁市| 连州市| 通许县| 南康市| 油尖旺区| 建水县| 昌邑市| 娱乐| 和田县| 望城县| 本溪市| 广宁县| 阿瓦提县| 临邑县| 玉树县| 辽中县| 水城县|