99超碰中文字幕在线观看-天天干天天日天天舔婷婷-我看操逼的好看的女人的-日本一二三四五区日韩精品

武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Isolation of Mice Muscle Cells

時間:2021-10-27 閱讀:145
分享:

PriCells: Isolation of Mice Muscle Cells                                                                       


The gastrocnemius, soleus, and plantaris were excised from three C57BL/6-Ly-5.1 6-week-old mice.
Tendons, all bone, and fat were carefully discarded, and the muscle tissue was thoroughly minced and then digested at 37°C with 0.2% collagenase for 45 min, followed by 0.1% trypsin for 45 min (PriCells Isolation Kit).
The tissue was triturated vigorously and passed through a 70-μm filter, and the cells were collected by centrifugation.
The cells were then plated in PriCells Culture System for 1 h at 37°C.
The nonadherent cells were then transferred to another plate, and the adherent cells (primarily fibroblasts) were discarded.
After 24 h, the floating cells and debris were washed off the plate, and fresh medium was applied to the attached cells.
After 5 days of culture, around 2 × 105 cells were collected from the plate after light trypsinization.

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~
撥打電話 產(chǎn)品分類
在線留言
高碑店市| 临夏县| 宣武区| 长海县| 会东县| 梁平县| 丽江市| 壤塘县| 年辖:市辖区| 扶风县| 沅陵县| 武穴市| 于田县| 故城县| 黄梅县| 永川市| 天全县| 西丰县| 鹰潭市| 大冶市| 金乡县| 六盘水市| 疏勒县| 犍为县| 夹江县| 安阳市| 万安县| 阿合奇县| 本溪市| 马关县| 泉州市| 乌拉特后旗| 兴业县| 宁陵县| 石屏县| 淮北市| 罗平县| 天柱县| 沁水县| 宜良县| 蛟河市|