CAS：100068-60-8

Molecular weight：1013.39

Extinction coefficient (cm -1 M -1)：2700001

Excitation (nm)：754

Emission (nm)：778

DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

          0.1 mg     0.5 mg       1 mg        5 mg      10 mg

1 mM   98.679 µL   493.393 µL   986.787 µL   4.934 mL   9.868 mL

5 mM   19.736 µL   98.679 µL   197.357 µL   986.787 µL   1.974 mL

10 mM   9.868 µL 49.339 µL   98.679 µL   493.393 µL   986.787 µL

染料DiI， DiO， DiD 和 DiR是一類親脂性熒光染料家族，用于標(biāo)記細(xì)胞膜和疏水性組織。這是一類環(huán)境敏感型熒光染料，當(dāng)它們與膜結(jié)合或者與親脂性生物分子（例如蛋白質(zhì)，雖然在水中其熒光強度很弱）結(jié)合時，其熒光強度顯著增強。它們具有很高的淬滅系數(shù)，偏光依賴性和很短的激發(fā)壽命。一旦應(yīng)用于細(xì)胞中，這種染料會在細(xì)胞內(nèi)質(zhì)膜中逐步擴(kuò)散，導(dǎo)致在其最佳濃度條件下，將整個細(xì)胞染色。它們不同的熒光顏色：DiI（橙色熒光）、DiO（綠色熒光）、DiD（紅色熒光）、DiR（深紅色熒光），為活細(xì)胞多色彩熒光成像分析和流式細(xì)胞術(shù)提供了一種便捷的工具。DiO和DiI可以分別與標(biāo)準(zhǔn)的FITC和TRITC濾光器一同使用。其中，DiO可以被633 nm He-Ne激光激發(fā)，并且具有比DiI更長的激發(fā)和發(fā)射光波長，為標(biāo)記細(xì)胞和組織的那些本身就具有本底熒光的染料提供了非常卓-越的替代品。DiR在活體成像或者示蹤中非常有用，因為它們所發(fā)射的紅外光可以高效地穿過細(xì)胞和組織，并且在紅外光范圍內(nèi)，其本底熒光水平很低。

艾美捷細(xì)胞膜熒光探針DiR（AAT-22070）基本參數(shù)：

中文名稱：細(xì)胞膜熒光探針DiR,碘化物

英文名字：DiR iodide[1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]

規(guī)格：25mg

光譜學(xué)相關(guān)參數(shù)：Ex=748nm / Em=780nm；其對應(yīng)分子量大小為1013。39MW。

形式：固體

保存建議：推薦使用常溫進(jìn)行運輸。建議在低于-15℃溫度下冷凍保存，要求儲存環(huán)境干燥并避免光照。

普通儲備溶液制備：

表1。將碘化二R（1,1-二十八烷基-3,3,3,3,3-四甲基吲哚三碳菁碘）的比質(zhì)量重建至給定濃度所需的DMSO體積。注意，體積僅用于制備儲備溶液。有關(guān)適當(dāng)?shù)膶嶒?/span>/生理緩沖液，請參考樣本實驗方案。

圖2：使用DiR（分類號22070）對活HeLa細(xì)胞質(zhì)膜進(jìn)行染色。圖1：使用DiR（22070）對活HeLa細(xì)胞質(zhì)膜進(jìn)行染色。細(xì)胞核用Hoechst 33342（17530）共染色。

細(xì)胞膜熒光探針DiR相關(guān)文獻(xiàn)：

1.親脂性近紅外染料在造血細(xì)胞歸巢全身光學(xué)成像中的應(yīng)用

作者：Kalchenko V、Shivtiel S、Malina V、Lapid K、Haramati S、Lapidot T、Brill A、Harmelin A。

期刊：J Biomed Opt（2006）：50507

2.Nox2、Ca2+和蛋白激酶C在血管緊張素II誘導(dǎo)的孤束核自由基產(chǎn)生中起作用

作者：Wang G、An寧J、Glass MJ、Tarsitano MJ、Zhou P、Frys KA、Pickel VM、Iadecola C。

雜志：高血壓（2006）：482

3.斑點海兔鼻柄的功能神經(jīng)解剖學(xué)

作者：Wertz A、Rossler W、Obermayer M、Bickmeyer U。

期刊：Front Zool（2006）：6