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Gibco/31800022/RPMI 1640 干粉培養(yǎng)基現(xiàn)貨

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31800022	RPMI 1640 Medium, powder
15260037	Bovine Albumin Fraction V (7.5% solution) (Gibco™)
17005042	Keratinocyte-SFM (1X) (Gibco™)
25200056	Trypsin-EDTA (0.25%), phenol red (Gibco™)
12585014	Insulin, human recombinant, zinc solution
12100046	DMEM, powder, high glucose
16010159	Newborn Calf Serum, New Zealand origin

 

描述

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
 

This RPMI is modified as follows:

With			Without
• L-glutamine			• HEPES
• Phenol Red			• Sodium Bicarbonate

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Product intended use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

 

規(guī)格

Glutamine:	L-Glutamine
Phenol Red Indicator:	Phenol Red
Form:	Powder
HEPES Buffer:	No HEPES
Serum Supplementation:	Standard Serum Supplementation
Sodium Pyruvate Additive:	No Sodium Pyruvate
Product Line:	Gibco®
Product Size:	10 x 1 L
Shipping Condition:	Room Temperature