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謝劍平院士：碳納米顆粒促煙草鉀吸收機(jī)制 | NMT煙草品質(zhì)創(chuàng)新科研平臺(tái)
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NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國(guó)15年。2020年，中國(guó)NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場(chǎng)。
研究使用平臺(tái)：NMT煙草品質(zhì)創(chuàng)新科研平臺(tái)
期刊：Journal of Nanobiotechnology
主題：碳納米顆粒促煙草鉀吸收機(jī)制
標(biāo)題：Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY?2 cells
影響因子：5.345
檢測(cè)指標(biāo)：K+流速
檢測(cè)樣品：煙草
NO3-流實(shí)驗(yàn)處理方法：
煙草BY-2懸浮細(xì)胞，分別在62.50μg/mL CNP，125.00μg/mL CNP和125.00μg/mL CNP+10mM TEA環(huán)境下處理12小時(shí)
NO3-流實(shí)驗(yàn)測(cè)試液成份：
46.88 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.9
作者：中國(guó)煙草總公司鄭州煙草研究院謝劍平、陳麗娟
中文摘要（谷歌機(jī)翻）
碳納米顆粒（CNPs）可以促進(jìn)植物生長(zhǎng)，而到目前為止，尚未報(bào)道CNPs促進(jìn)植物生長(zhǎng)吸收鉀的機(jī)制。
在這項(xiàng)研究中，建立了CNPs促進(jìn)BY-2細(xì)胞吸收鉀的功能，并且細(xì)胞中積累的鉀與細(xì)胞的新鮮生物量具有顯著相關(guān)性。細(xì)胞中的K+隨著CNP濃度的增加而增加。用CNP處理后，K+流入量達(dá)到高水平，并且顯著高于用K+阻斷劑氯化四乙銨（TEA+）處理的對(duì)照組和陰性組。
在CNPs抑制劑存在下，K+積累沒有減少。與對(duì)照組相比，在鉀通道阻滯劑TEA+或CNPs抑制劑的存在下，NKT1基因表達(dá)發(fā)生了變化。發(fā)現(xiàn)CNP比圓錐形納米毛細(xì)管中通過離子電流測(cè)定法（RIC）整流確定的其他陽(yáng)離子優(yōu)先運(yùn)輸K+。
這些結(jié)果表明CNPs上調(diào)鉀基因表達(dá)以增強(qiáng)BY-2細(xì)胞中的K+。此外，據(jù)推測(cè)，CNP通過大量的羧基模擬了離子通道的蛋白質(zhì)，以滲透K+。這些發(fā)現(xiàn)將為通過碳納米顆粒改善植物生長(zhǎng)提供支持。
Effects of CNPs and inhibitor treatments on net K+ fluxes in BY-2 cells. a K fluxes were determined with after different CNPs treatments lasting for 900 s measurements. b Schematic diagram of test sample. c Mean value of K+ fluxes at different concentration of CNPs in cells. Group 1: 62.50 μg/mL CNPs, Group 2: 125.00 μg/mL CNPs, Group 3: 125.00 μg/mL CNPs + 10 mM TEA+. The mean value of each group contains six individual cell, and error bars represent SE, n = 6. Asterisk (*) indicates significant difference compared with control group (p < 0.05)
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英文摘要
Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth has not been reported so far.
In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of cells. The K+ in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ blocker, tetraethylammonium chloride ( TEA+).
The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary.
These results indicated that CNPs upregulated potassium gene expression to enhance K+ in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles.