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NMT歷*的今天丨胞外Ca2+內(nèi)流對(duì)松果花粉管發(fā)育影響的蛋白質(zhì)組學(xué)
NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國(guó)15年。2020年，中國(guó)NMT銷往瑞士蘇黎世大學(xué)，正式打開(kāi)歐洲市場(chǎng)
NMT歷*的今天
2008年04月01日，中科院植物研究所林金星、吳小琴用NMT在Journal of Proteome Research上發(fā)表了標(biāo)題為Integrative proteomic and cytological analysis of the effects of extracellular Ca2+ influx on Pinus bungeana pollen tube development的研究成果。
期刊：J Proteome Res
主題：胞外Ca2+內(nèi)流對(duì)松果花粉管發(fā)育影響的蛋白質(zhì)組學(xué)和細(xì)胞學(xué)綜合分析
標(biāo)題：Integrative proteomic and cytological analysis of the effects of extracellular Ca2+ influx on Pinus bungeana pollen tube development
影響因子：5.684
檢測(cè)指標(biāo)：Ca2+流速
作者：中科院植物研究所林金星、吳小琴
英文摘要
Ca2+ is an essential ion in the control of pollen germination and tube growth. However, the control of pollen tube development by Ca2+ signaling and its interactions with cytoskeletal components, energy-providing pathways, and cell-expansion machinery remain elusive. Here, we used nifedipine (Nif) to study Ca2+ functions in differential protein expression and other cellular processes in Pinus bungeana pollen tube growth.
Proteomics analysis indicated that 50 proteins showed differential expression with varying doses of Nif. Thirty-four of these were homologous to previously reported proteins and were classified into different functional categories closely related to tip-growth machinery.
Blocking the L-type Ca2+ channel with Nif in the pollen tube membrane induced several early alterations within a short time, including a reduction of extracellular Ca2+ influx and a subsequently dramatic decrease in cytosolic free Ca2+ concentration ([Ca2+]c), concomitant with ultrastructural abnormalities and changes in the abundance of proteins involved in energy production and signaling. Secondary alterations included actin filament depolymerization, disrupted patterns of endocytosis/exocytosis, and cell wall remodeling, along with changes in the proteins involved in these processes.
These results suggested that extracellular Ca2+ influx was necessary for the maintenance of the typical tip-focused [Ca2+]c gradient in the P. bungeana pollen tube, and that reduced adenosine triphosphate production (ATP), depolymerization of the cytoskeleton, and abnormal endocytosis/exocytosis, together with enhanced rigidity of cell walls, were responsible for the growth arrest observed in pollen tubes treated with Nif.
中文摘要（谷歌機(jī)翻）
Ca2+是控制花粉萌發(fā)和管生長(zhǎng)的必需離子。但是，通過(guò)Ca2+信號(hào)傳導(dǎo)控制花粉管發(fā)育及其與細(xì)胞骨架成分，能量提供途徑和細(xì)胞擴(kuò)增機(jī)制的相互作用仍然難以捉摸。在這里，我們使用硝苯地平（Nifdipine）研究松果花粉花粉管生長(zhǎng)中Ca2+在差異蛋白表達(dá)和其他細(xì)胞過(guò)程中的功能。
蛋白質(zhì)組學(xué)分析表明，隨著Nif劑量的變化，50種蛋白質(zhì)表現(xiàn)出差異表達(dá)。其中的34個(gè)與先前報(bào)道的蛋白質(zhì)同源，并被歸類為與生長(zhǎng)機(jī)制密切相關(guān)的不同功能類別。
在花粉管膜中用Nif阻斷L型Ca2+通道可在短時(shí)間內(nèi)引起數(shù)個(gè)早期變化，包括細(xì)胞外Ca2+內(nèi)流減少和隨后胞漿游離Ca2+濃度（[Ca2+] c）急劇下降，并伴有超微結(jié)構(gòu)能量產(chǎn)生和信號(hào)傳導(dǎo)中涉及的蛋白質(zhì)異常和豐度變化。次要的變化包括肌動(dòng)蛋白絲解聚，內(nèi)吞/胞吐作用的破壞模式，細(xì)胞壁重塑以及這些過(guò)程中涉及的蛋白質(zhì)變化。
這些結(jié)果表明，胞外Ca2+內(nèi)流對(duì)于維持P. bungeana花粉管中典型的集中[Ca2+] c梯度是必要的，并且減少了三磷酸腺苷的產(chǎn)生（ATP），細(xì)胞骨架的解聚以及異常的內(nèi)吞/胞吐作用以及細(xì)胞壁的剛性增強(qiáng)是造成在用Nif處理的花粉管中觀察到的生長(zhǎng)停滯的原因。
Figure 1. Dose-dependent inhibitory effects of exogenous Nif on extracellular Ca2+in?ux at the pollen tube tips. A typical extracellular Ca2+ in?ux can be detected in the very tip of control pollen tube, and 100 µM Nif treatment decreased the Ca2+ in?ux, while 250 and 500 µM Nif treatment greatly reduced this Ca2+ in?ux.