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林木耐旱創(chuàng)新科研平臺(tái)： 轉(zhuǎn)錄因子調(diào)控楊樹(shù)根系生長(zhǎng)影響抗旱
NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國(guó)15年。2020年，中國(guó)NMT銷往瑞士蘇黎世大學(xué)，正式打開(kāi)歐洲市場(chǎng)。
研究使用平臺(tái)：NMT林木耐旱創(chuàng)新科研平臺(tái)
期刊：New Phytologist
主題：轉(zhuǎn)錄因子PdNF-YB21調(diào)控楊樹(shù)根系生長(zhǎng)并影響抗旱性
標(biāo)題：The root-specific NF-Y family transcription factor, PdNF-YB21, positively regulates root growth and drought resistance by ABA-mediated IAA transport in Populus
影響因子：7.299
檢測(cè)指標(biāo)：H+
檢測(cè)樣品：楊樹(shù)根
H+流實(shí)驗(yàn)處理方法：
楊樹(shù)莖段在Hoagland營(yíng)養(yǎng)液/Hoagland營(yíng)養(yǎng)液+0.1μM ABA/Hoagland營(yíng)養(yǎng)液+10μM FLU培養(yǎng)30天后檢測(cè)根。
H+流實(shí)驗(yàn)測(cè)試液成份：未寫明
作者：北京林業(yè)大學(xué)夏新莉、周揚(yáng)顏
中文摘要（谷歌機(jī)翻）
根系生長(zhǎng)控制在植物對(duì)干旱脅迫的適應(yīng)中起著重要作用，但是這種控制的潛在分子機(jī)制仍然很難捉摸。
在此，從胡楊中分離出根特異性核因子Y（NF-Y）轉(zhuǎn)錄因子PdNF-YB21。PdNF-YB21的功能機(jī)制通過(guò)各種形態(tài)，生理，分子，生化和光譜學(xué)技術(shù)進(jìn)行了表征。
楊樹(shù)中PdNF-YB21的過(guò)表達(dá)促進(jìn)木質(zhì)部血管高度木質(zhì)化和擴(kuò)大的根系生長(zhǎng)，從而增加了抗旱性。相反，CRISPR / Cas9介導(dǎo)的楊樹(shù)突變體nf-yb21表現(xiàn)出降低的根系生長(zhǎng)和抗旱性。PdNF-YB21與B3域轉(zhuǎn)錄因子PdFUSCA3（PdFUS3）相互作用。PdFUS3直接激活A(yù)BA合成關(guān)鍵基因PdNCED3的啟動(dòng)子，導(dǎo)致水分缺乏的楊樹(shù)根ABA含量顯著增加。楊NF-YB21和FUS3的共表達(dá)顯著增強(qiáng)了PdNCED3的表達(dá)。
此外，ABA促進(jìn)了IAA在根尖的轉(zhuǎn)運(yùn)，終增加了根的生長(zhǎng)和抗旱性。
英文摘要
Root growth control plays an important role in plant adaptation to drought stress, but the underlying molecular mechanisms of this control remain largely elusive.
Here, a root-specific nuclear factor Y (NF-Y) transcription factor PdNF-YB21 was isolated from Populus. The functional mechanism of PdNF-YB21 was characterized by various morphological, physiological, molecular, biochemical, and spectroscopy techniques.
Overexpression of PdNF-YB21 in poplar promoted root growth with highly lignified and enlarged xylem vessels, resulting in increased drought resistance. In contrast, CRISPR/Cas9-mediated poplar mutant nf-yb21 exhibited reduced root growth and drought resistance. PdNF-YB21 interacted with PdFUSCA3 (PdFUS3), a B3 domain transcription factor. PdFUS3 directly activated the promoter of the ABA synthesis key gene PdNCED3, resulting in a significant increase in root ABA content in poplars subjected to water deficit. Coexpression of poplar NF-YB21 and FUS3 significantly enhanced the expression of PdNCED3.
Furthermore, ABA promoted IAA transport in root tips, which ultimately increased root growth and drought resistance.