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J Agr Food Chem：湖南農(nóng)大 | 低氮提升油菜氮利用率
本文作者吳智敏文章解讀
期刊：JOURNAL OF AGRICULTURALAND FOOD CHEMISTRY
主題：低氮提升油菜氮利用率新機制
標題：Low Nitrogen Enhances Nitrogen Use Efficiency by Triggering NO3– Uptake and Its Long-Distance Translocation
影響因子：3.412
檢測指標：NO3-、H+
作者：湖南農(nóng)業(yè)大學張振華、吳智敏
英文摘要
Nitrogen is essential for plant growth andcrop productivity; however, nitrogen use e?ciency (NUE) decreases withincreasing N supply, resulting in a waste of resources. Molecular mechanismsunderlying low-nitrogen (LN)-mediated enhancement of NUE are not clear.
We used high-NUE Brassica napus genotype H(Xiangyou 15), low-NUE B. napus genotype L (814), and Arabidopsis mutant aux1to elucidate the mechanism underlying the changes in NUE under di?erent ratesof N fertilizer application. NUE of B. napus increased under LN, which enhancedN uptake ability by regulating root system architecture and plasma membraneH+-ATPase activity; AUX1 was involved in this process.
Additionally, BnNRT1.5 was upregulated andBnNRT1.8 was downregulated under LN, whereby more N was transferred to theshoot through enhanced N transport. Observed changes in photosynthesis under LNwere associated with N assimilation e?ciency. Our study provides new insightsinto the mechanisms of plant adaptation to the environment.
中文摘要（谷歌機翻）
氮對植物生長和作物生產(chǎn)力至關重要；然而，隨著氮供應的增加，氮利用效率（NUE）降低，導致資源浪費。低氮（LN）介導的NUE增強的分子機制尚不清楚。
我們使用高NUE甘藍型油菜基因型H（香優(yōu)15），低NUE B.napus基因型L（814）和擬南芥突變體aux1來闡明在不同氮肥施用量下NUE變化的機制。甘藍型油菜NUE在LN下增加，通過調節(jié)根系結構和質膜H + -ATPase活性提高N吸收能力; AUX1參與了這個過程。
另外，BnNRT1.5被上調并且BnNRT1.8在LN下被下調，由此通過增強的N轉運將更多的N轉移到芽。觀察到的LN下光合作用的變化與N同化效率有關。我們的研究為植物適應環(huán)境的機制提供了新的見解。
Gas exchange, chlorophyll content, and photosynthetic N use in two contrasting B. napus genotypes for N use e?ciency under LN (1 mM NO3− N, black bars) or CK (15 mM NO3− N, white bars) conditions: (a) photosynthetic rate, (b) SPAD, (c) transpiration rate, and (d) photosynthetic N use e?ciency (PNUE). The bar height represents the mean, and error bars indicate standard deviation (n = 6). Di?erent letters on bars indicate signi?cant di?erences based on Tukey’s HSD (p < 0.05). The statistical analysis was performed on di?erent treatments within genotypes.
本課題組往期NMT文章：
· V-ATPase and V-PPase at the Tonoplast Affect NO3- Content in Brassica napus by Controlling Distribution of NO3- Between the Cytoplasm and Vacuole
· Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus
· NRT1.1-related NH4+ toxicity is associated with balance between NH4+ uptake and assimilation
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