化工儀器網(wǎng)
資料下載
滬鼎產(chǎn)品文獻(xiàn):魚PPAR-α,MTP等ELISA試劑盒引用文獻(xiàn)
閱讀：987 發(fā)布時間：2021-7-23| 提 供 商 | 上海滬鼎生物科技有限公司 | 資料大小 | 777.5KB |
|---|---|---|---|
| 資料圖片 | 下載次數(shù) | 62次 | |
| 資料類型 | PDF 文件 | 瀏覽次數(shù) | 987次 |
| 免費(fèi)下載 | 點(diǎn)擊下載 |
【文獻(xiàn)標(biāo)題】Replacing fish oil with palm oil: Effects on mRNA expression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia (Oreochromis niloticus)
【作者】Christian Larbi Ayisi,Jin-Liang Zhao,Xue-Ming Hua,et.al
【作者單位】上海海洋大學(xué)（Shanghai Ocean University）
【文獻(xiàn)中引用產(chǎn)品】
魚過氧化物酶體增殖物激活受體α(PPAR-α)ELISA試劑盒
魚過氧化物酶體增殖物激活受體β(PPARβ)ELISA試劑盒
魚脂蛋白酯酶（LPL）ELISA試劑盒
魚脂肪酸結(jié)合蛋白3（FABP3）ELISA試劑盒
魚金屬忍耐蛋白(MTP)ELISA試劑盒
魚可溶性肝X受體(LXR)ELISA試劑盒
魚脂肪酸延長酶5(ELOVL5)ELISA試劑盒
魚葡萄糖6磷酸脫氫酶(G6PD)ELISA試劑盒
魚蘋果酸酶（ME）ELISA試劑盒
【關(guān)鍵詞】ELOVL 5, fatty acid metabolism, fatty acid transport, glucose 6-phosphate dehydrogenase,
lipoprotein lipase, palm oil
【DOI】doi.org/10.1111/anu.12821
【影響因子(IF)】2.0
【出版期刊】《Aquaculture Nutrition》
【產(chǎn)品原文引用】
Enzyme activities
Enzyme-linked immunosorbent assay (ELISA) was used to measure the enzyme activities of PPAR-α, PPAR-β, LPL, FABP-3, MTP, LXR, ELOVL 5, 6GPD, G6PD and ME with a commercial kit from Shanghai Ding Biological Technology Company Limited (Shanghai, China). A ground glass homogenizer was used to homogenize liver (0.5-1.0 g) on ice. The homogenates were centrifuged (110 000 g, 20 min at 4°C), and the clear phase between the top layer and the pellets was used for the analysis. At 450 nm, an ELISA microplate reader was used to measure the optical density (OD) of samples in a 96-well plate. According to the manufacturer’s instructions, a standard curve was generated and the standard diversity calculated using Excel 2003. Enzyme activity units (IU), defined as moles of substrate, were converted into product per minute at assay temperature.
完整版PDF文獻(xiàn)請咨詢在線客服或者dianhua聯(lián)系我司業(yè)務(wù)員！
更多公司福利請關(guān)注“上海滬鼎"微信公眾號！
