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Zeocin博萊霉素 優(yōu)勢(shì)產(chǎn)品
點(diǎn)擊次數(shù)：3645 發(fā)布時(shí)間：2016-3-30
Zeocin博萊霉素 優(yōu)勢(shì)產(chǎn)品
產(chǎn)品簡(jiǎn)介
Zeocin™ 試劑是博來霉素抗生素家族的一個(gè)成員。其抗性由編碼一種 13,665 道爾頓蛋白質(zhì)的 Sh ble 基因賦予。在表達(dá)這種蛋白質(zhì)的細(xì)胞中，Zeocin™ 無法結(jié)合和切割細(xì)胞 DNA。 Zeocin™ 對(duì)大多數(shù)好氧細(xì)胞有效，可用于選擇哺乳動(dòng)物和昆蟲細(xì)胞系、酵母以及細(xì)菌。 選擇細(xì)胞時(shí)，Zeocin™ 的用量為50-2000 ug/ml（通常 300 ug/ml），具體取決于細(xì)胞類型。
C55H83N19O21S2Cu
式量： 1137.41 克/摩爾
Zeocin™ 是 CAYLA 的商標(biāo)。
ble 抗性基因受一個(gè)或多個(gè)美國（號(hào) 5,021,344 和 5,118,620）以及相關(guān)外國保護(hù)，Cayla 擁有權(quán)，Invitrogen 已從 Cayla 獲得使用許可。 該產(chǎn)品的銷售于研究目的。
使用:
●液體Zeocin可用于細(xì)胞培養(yǎng)。
●用于篩選轉(zhuǎn)化株的Zeocin濃度根據(jù)pH值和鹽濃度改變。
●pH越高，鹽濃度越低Zeocin活性越大。
●普通大腸桿菌菌株篩選可在25＆micro；g／ml zeocin濃度的低鹽LB瓊脂培養(yǎng)基中進(jìn)行，pH調(diào)至7.5。
●動(dòng)物細(xì)胞篩選：根據(jù)不同細(xì)胞系從100到750＆micro；g／ml。
●植物細(xì)胞篩選：根據(jù)不同的細(xì)胞種類和培養(yǎng)基從50到250＆micro；g／ml。
●酵母篩選：取決于種屬和培養(yǎng)基，S。cerevisiaeSh ble轉(zhuǎn)化株在50＆micro；g／ml濃度的YPD培養(yǎng)基中篩選，pH7.0.304015.
Invitrogen相關(guān)資料如下:
Zeocin™ Selection Reagent
Cat. No. R250-01
Description
Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistanceis conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cellsexpressing the protein, Zeocin™ is prevented frombinding and cleaving the cellular DNA. Zeocin™ iseffective on most aerobic cells and can be used for selection in mammalian andinsect cell lines, yeast, and bacteria. The amount of Zeocin™required for selection will vary between 50-2000 ug/ml (typically300 ug/ml) depending on the cell type.
C55H83N19O21S2Cu
Formula Weight: 1137.41 g/mole
Zeocin™ is a trademark of CAYLA.
The ble resistance gene is the subject ofone or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreignequivalents owned by Cayla and licensed to Invitrogen. This product is sold forresearch purposes only. For commercial license information, please contact:Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA92008.
General Specifications
Concentration: 100 mg⁄mL
Brand: Zeocin™
Form: Liquid
Product Size: 1 g
ValidatedApplication: Eukaryotic Selection⁄Stable Cell Line Generation
Bacterial Selection
Contents & Storage
Zeocin™ is provided in solution at a concentration of 100 mg/ml. The 1 gramsize is supplied in 8 vials. The 5 gram size is supplied in one vial. Store Zeocin™ at -20°C inthe dark. Zeocin™ is guaranteed stable for 6 months when properly stored.
品名 | 產(chǎn)地 | 貨號(hào) | 規(guī)格 | 單價(jià) | 備注 |
Invitrogen | R25001 | 8x0.125ml | 詢價(jià) | 現(xiàn)貨 |