genelink 40-5131-05說(shuō)明書(shū)

產(chǎn)品目錄號(hào) 40-5131-05

產(chǎn)品名稱(chēng) LiCl RNA 沉淀溶液 (7.5M LiCl，50 mM EDTA pH 8.0)；50 mL

Size50 mL

DescriptionLiCl RNA 沉淀溶液 (7.5M LiCl，50 mM EDTA pH 8.0)；50 mLDo 不用于小于 300 個(gè)核苷酸的 RNA 沉淀。終濃度為 2.5M 的 LiCl 在不添加乙醇的情況下可有效沉淀大于 300 個(gè)核苷酸的 RNA，這種沉淀方法選擇性沉淀 RNA，不能有效沉淀 DNA、蛋白質(zhì)或碳水化合物。它是從 RNA 制劑中去除翻譯或 cDNA 合成抑制劑的優(yōu)選方法。它還提供了一種從體外轉(zhuǎn)錄反應(yīng)中回收 RNA 的簡(jiǎn)單快速方法。在 4 °C 下以 12K rpm 離心 20 min，可定量回收低至 50 ng 的 RNA。

用途：2.5 M 氯化鋰終濃度，不添加乙醇或異丙醇。

濃度≥100 ng/mL 時(shí) rna 的常規(guī)沉淀。

Catalog Number

40-5131-05

Product Name

LiCl RNA Precipitation Solution (7.5M LiCl, 50 mM EDTA pH 8.0); 50 mL

Size

50 mL

Description

LiCl RNA Precipitation Solution (7.5M LiCl, 50 mM EDTA pH 8.0); 50 mL

Do not use for precipitation of RNA smaller than 300 nucleotides.

A final concentration of 2.5M LiCl is effective in precipitation of RNA larger than 300 nucleotides without the addition of

ethanol, this method of precipitation selectively precipitates RNA and does not efficiently precipitate DNA, protein or

carbohydrate. It is the method of choice for removing inhibitors of translation or cDNA synthesis from RNA preparations. It

also provides a simple rapid method for recovering RNA from in vitro transcription reactions. As little as 50 ng of RNA can be

quantitatively recovered by centrifugation at 12K rpm for 20 minutes at 4°C.

Usage: 2.5M Lithium Chloride final concentration without addition of ethanol or isopropanol. Routine precipitation of

RNA at Concentrations ≥100 ng/mL.