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【文獻標(biāo)題】Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

【作者】Demin Feng，Hui Huang，Yang Yang，et.al

【作者單位】鄭州大學(xué)（Zhengzhou University）

【文獻中引用產(chǎn)品】

大鼠睪酮（T）ELISA試劑盒

【關(guān)鍵詞】Sodium fluoride，Oxidative stress，DNA damage，N-acetylcysteine，Spermatogenesis

【DOI】dx.doi.org/10.1016/j.mrgentox.2015.09.004

【影響因子(IF)】2.32

【出版期刊】《Mutation Research-Genetic Toxicology and Environmental Mutagenesis》

【產(chǎn)品原文引用】

2.6. Determination of testosterone levels

The serum levels of testosterone were assayed by the method of double- antibody sandwich ELISA using rat-specific ELISA Kits (HengYuan Biotech Co., Ltd, ShangHai, China). All the operations followed the protocols provided in the kits. The sensitivity of the assays for testosterone was 0.5 nmol/L and the detectable range was 5 nmol/L–160 nmol/L. All samples were measured in duplicate and run in a single assay, which had an inter-assay coefficient of variation (CV) of 9.0% and an intra-assay CV of 11.0%.

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