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Mouse Anti- ER
Catalog Number : HZ80056 Quantity size : 0.1ml （dilute with pH 7.4
0.01 M PBS or antibody diluent ）
Background: Estrogen receptors （ER） are members of the steroid/thyroid hormone
receptor superfamily of ligand-activated transcription factors. Estrogen receptors,
including ERα and ERβ, contain DNA- and ligand-binding domains and are critically
involved in regulating the normal function of reproductive tissues. They are located in
the nucleus, though some estrogen receptors associate with the cell surface
membrane and can be rapidly activated by exposure of cells to estrogen. ERα and
ERβ have been shown to be differentially activated by various ligands.
Receptor-ligand interactions trigger a cascade of events, including dissociation from
heat shock proteins, receptor dimerization, phosphorylation and the association of the
hormone-activated receptor withspecific regulatory elements in target genes.
Evidence suggests that Erα and ERβ may be regulated by distinct mechanisms, even
though they share many functional characteristics.
Specificity :
– ER is a mouse monoclonal antibody.
– specific for ER antibody of human
– use for western blotting, elisa, immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 56kDa.
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for
greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M
PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃.
Important Note: This product as supplied is intended for research use only, not for
use in human, therapeutic or diagnostic applications.