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技術(shù)文獻(xiàn):植物IAA,GA,PEPC,AGPase,等試劑盒引用文獻(xiàn)
閱讀：1051 發(fā)布時(shí)間：2021-1-15【文獻(xiàn)題目】 Water Polishing improved controlled-release characteristics and fertilizer efciency of castor oil-based polyurethane coated diammonium phosphate
【作者】 Hao Lu, HongyuTian, Min Zhang，et al.
【作者單位】 山東農(nóng)業(yè)大學(xué)
【引用試劑盒】
植物赤霉素4（GA4）ELISA試劑盒
植物赤霉素12（GA12）ELISA試劑盒
植物生長(zhǎng)素（IAA）ELISA試劑盒
植物磷酸烯醇式丙酮酸羧化酶（PEPC）ELISA試劑盒
植物ADPG焦磷酸化酶(AGPP)ELISA試劑盒
植物磷酸核糖焦磷酸酰轉(zhuǎn)移酶(PRPP)Elisa試劑盒
植物腺苷酸代琥珀酸合成酶(AMPSS)elisa試劑盒
植物赤霉素(GA)ELISA試劑盒
植物腺苷二磷酸葡萄糖焦磷酸化酶（AGPase）ELISA檢測(cè)試劑盒
植物磷酸核糖焦磷酸?；D(zhuǎn)移酶(PRPPAT)ELISA試劑盒
【影響因子（IF）】 4.29
【DOI】 doi.org/10.1038/s41598-020-62611-w
【出版期刊】《SCIENTIFIC REPORTS》
【產(chǎn)品引用原文】
Pot experiment.
Four treatments were carried out to verify the fertilizer efciency of CDAP:
(1) without phosphate fertilizer (P0);
(2) DAP (P1);
(3) 40% CDAP with 60% DAP (C40P1);
(4) 60% CDAP with 40% DAP (C60P1).
Te amount of phosphate ertilizer applied to all treatments with phosphate fertilizer was 3.2 g P2O5 pot-¹. Te CDAP used in the pot experiment was WAT2 with 3% coating materials. Except for DAP and CDAP, the rest of nitrogen and phosphate fertilizer was supplied by common urea and calcium superphosphate, respectively. Te nitrogen application amount of all treatments was 3.2 gN pot-¹. Te application rate of potassium is 1.6 g K2O pot-¹ as potassium chloride. All fertilizers were mixed evenly in 15 kg soil and put into a pot
with 10 kg sand at the bottom.
Auxin (IAA), gibberellin (GA), phosphoenolpyruvate carboxylase (PEPC), ADP-glucosepyrophosphorylase (AGPase), phosphoribosyl pyrophosphate (PRPP),adenosine monophosphate synthetase (AMPSS) and phosphoribosyl pyrophosphate aminotransferase (PRPPAT)were extracted and assayed with a ELISA kit from Shanghai HengYuan Biological Technology Co. Ltd. (Shanghai, China). Soil available P content was extracted with 0.5 mol L-¹ sodium bicarbonate solution, and the absorbance was measured by spectrophotometer afer molybdenum and antimony. Phosphorus use efciency (PUE)=(P accumulation in P area - accumulation of P
in blank area)/P application rate × 100%
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