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99KB |
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SMD1163
| 型號 | 載體名稱 | 出品公司 | 載體用途 |
| VBI0307 | SMD1163 | Invitrogen | 配套畢赤酵母 |
Introduction
Pichia pastoris is a highly successful system for production of a wide variety of
recombinant proteins. Several factors have contributed to its rapid acceptance, the most
important of which include: （1） a promoter derived from the alcohol oxidase I （AOX1） gene of P.
pastoris that is uniquely suited for the controlled expression of foreign genes; （2） the similarity
of techniques needed for the molecular genetic manipulation of P. pastoris to those of
Saccharomyces cerevisiae; （3） the strong preference of P. pastoris for respiratory growth, a key
physiological trait that greatly facilitates its culturing at high-cell densities relative to
fermentative yeasts; and （4） the decision in 1993 by Phillips Petroleum Company in Bartlesville,
Okla. （and continued by Research Corporation Technologies [RCT]）, to release the P. pastoris
expression system to academic research laboratories, the consequence of which has been an
explosion in the knowledge base on the system as described in numerous recent publications.
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