pGAPZα C

型號(hào)	載體名稱	出品公司	載體用途
VJI0295	pGAPZα C	Invitrogen	酵母表達(dá)載體

The glyceraldehyde-3-phosphate dehydrogenase （GAPDH） enzyme is constitutively

expressed at high levels in many organisms, including Pichia pastoris. The promoter of

the gene （GAP） encoding the GAPDH protein has recently been characterized and shown

to express recombinant proteins to high levels in Pichia pastoris, depending on the

carbon source used （Waterham et al., 1997）. The level of expression seen with the GAP

promoter （PGAP） can be slightly higher than that obtained with the AOX1 promoter.

The pGAPZ A, B, and C vectors （2.9 kb） and pGAPZα A, B, and C （3.1 kb） vectors use

the GAP promoter to constitutively express recombinant proteins in Pichia pastoris.

Proteins can be expressed as fusions to a C-terminal peptide containing the myc epitope

for detection and a polyhistidine tag for purification on metal-chelating resin （i.e.

ProBondô）. In addition, pGAPZα produces proteins fused to an N-terminal peptide

encoding the Saccharomyces cerevisiae α-factor secretion signal. Both vectors are

supplied in three reading frames to facilitate in frame cloning with the C-terminal tag

and/or the N-terminal secretion signal. Selection of these vectors is based on the dominant

selectable marker, Zeocinô, which is bifunctional in both Pichia and E. coli.

質(zhì)粒圖譜:

上海起福生物科技有限公司

：楊建輝

：

辦公：

*8006

:http://www.qfbio.com/

地址：上海市浦東新區(qū)繡川路561號(hào)1102室

免費(fèi)下載

會(huì)員登錄