pTK-hyg

型號(hào)	載體名稱	出品公司	載體用途
VSC0467	pTK-hyg	Clontech	四環(huán)素調(diào)控系統(tǒng)

Description

pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the

selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable

cell lines using the Tet-On® or Tet-Off® Gene Expression Systems because the absence of an

enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived

plasmids upon cointegration into the host cell's genome.

Use

pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains

a gene of interest using any standard transfection technique. Most mammalian cell lines

require 200 µg/ml of Hygromycin to select for stably transformed cells.

Location of Features

• HSV TK polyA signals: 1473–1478 & 1486–1491 （complementary）

• Hygr

（hygromycin resistance gene）: 1537–2574 （complementary）

• P

HSV TK （HSV TK promoter）: 2588–2835 （complementary）

• pUC origin ofreplication: 3152–3795

• Ampr

（ampicillin resistance gene; beta-lactamase）: 3943–4803

Propagation in E. coli

• Suitable host strains: DH™ and other general purpose strains.

• Selectable marker: plasmid confers resistance to ampicillin （50 µg/ml） on E. coli hosts.

• E. coli replication origin: pUC

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