Quantity	50 µg
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG3
Conjugate	Unconjugated
Application	Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification	Affinity chromatography
Reactivity	AAV2, AAV2 7m8, AAV3
No reactivity	AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74
Storage before reconstitution	2-8°C until indicated expiry date
Storage after reconstitution	2-8°C
Intended use	Research use only
Clone	A20
Immunogen	AAV2 capsids
Concentration	50 µg/ml after reconstitution with 1 ml dist. water
Formulation	Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Binding affinity	KD value (AAV2) = 2.6E-11 M KD value (AAV3) = <1.0E-12 M
Synonym	Adeno-associated virus 2; AAV-2

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Affinity Chromatography	Assay dependent
Dot Blot	1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA	Assay dependent
Immunoprecipitation (IP)	1:5
Neutralization Assay	EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) - assay dependent

用于表征感染的不同階段，對分析AAV2組裝過程非常有用。A20與AAV2和AAV3、空衣殼和全衣殼特異性反應(yīng)。識別組裝衣殼的構(gòu)象表位，不存在于變性衣殼蛋白和天然但未組裝的衣殼蛋白中。該抗體不能用于免疫印跡。

表位定位實驗（Wobus等人，2000）確定了四個免疫反應(yīng)（不連續(xù)）區(qū)域。主要反應(yīng)歸因于AAV2衣殼的AA369至aa378序列。該抗體也可用于中和實驗。

Wobus，C.E.等。針對腺相關(guān)病毒2型（AAV-2）衣殼的單克隆抗體：參與AAV-2細胞相互作用和中和AAV-2感染的衣殼結(jié)構(gòu)域的表位定位和鑒定。J.維羅爾。74, 9281-93 (2000).

Moskalenko，M.等。人類抗腺相關(guān)病毒2型中和抗體的表位定位：對Ge的意義

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

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