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Anti-Pro-SP-C - Rabbit, n-terminal (WRAB-9337)使用說明
產(chǎn)品信息
品牌 | Seven Hills Bioreagents |
貨號 | WRAB-9337 |
規(guī)格 | 100 µL |
宿主 | 兔 |
反應(yīng)種屬 | 人、鼠 |
克隆類型 | 多克隆 |
偶 聯(lián) 物 | 無 |
緩沖物 | 0.01% sodium azide |
抗原 | 重組鼠肺表面活性蛋白C前體pro-SP-C, n-terminal (aa1-35) GST融合蛋白 |
保存條件 | 儲(chǔ)存在-20℃，避免反復(fù)凍融 |
產(chǎn)品使用
實(shí)驗(yàn)應(yīng)用 | 建議稀釋倍數(shù) |
Western Blot | 1:5000 |
Immunohistochemistry | 1:3000 |
說明：最佳稀釋倍數(shù)與濃度應(yīng)由研究人員確認(rèn)
應(yīng)用實(shí)例
圖片為成年小鼠肺組織用抗N-末端proSP-C兔血清（1:3000稀釋）染色。ProSP-C通過DAB（黑色）進(jìn)行可視化，并用Nuclear Fast Red（粉紅色）進(jìn)行復(fù)染。放大倍數(shù)40倍。
參考文獻(xiàn)
1. Glasser SW, Detmer EA, Ikegami M, Na CL, Stahlman MT, Whitsett JA. 'Pneumonitis and emphysema in sp-C gene targeted mice.' J Biol Chem. 2003 Apr 18;278(16):14291-8.
2. Nogee LM, Dunbar AE 3rd, Wert SE, Askin F, Hamvas A, Whitsett JA. 'A mutation in the surfactant protein C gene associated with familial interstitial lung disease.' N Engl J Med. 2001 Feb 22;344(8):573-9.
3. No abstract available. Glasser SW, Burhans MS, Korfhagen TR, Na CL, Sly PD, Ross GF, Ikegami M, Whitsett JA. 'Altered stability of pulmonary surfactant in SP-C-deficient mice.' Proc Natl Acad Sci U S A. 2001 May 22;98(11):6366-71.
4. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
5. Yang, L., J. Johansson, R. Ridsdale, H. Willander, M. Fitzen, H. T. Akinbi, and T. E. Weaver. Surfactant Protein B Propeptide Contains a Saposin-Like Protein Domain with Antimicrobial Activity at Low pH. J Immunol 2010;184:975-983.
6. Conkright, J. J., K. S. Apsley, E. P. Martin, R. Ridsdale, W. R. Rice, C. L. Na, B. Yang, and T. E. Weaver. Nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-C. Am J Respir Cell Mol Biol 2010;42:181-189.
7. Wang, M., J. P. Bridges, C. L. Na, Y. Xu, and T. E. Weaver. Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C. J Biol Chem 2009;284:33377-33383.
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