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zen-bio AM-1-L1說明書
Mouse 3T3-L1 Cell Line Systems
ZenBio現(xiàn)在提供冷凍保存和培養(yǎng)的3T3-L1前脂肪細(xì)胞！
30多年來，3T3-L1小鼠脂肪細(xì)胞一直是代謝疾病研究的基礎(chǔ)。3T3-L1系統(tǒng)最初由哈佛醫(yī)學(xué)院的Howard Green博士從瑞士小鼠胚胎組織中提取出來，在促進(jìn)對(duì)糖尿病、肥胖癥和相關(guān)疾病相關(guān)的基本細(xì)胞機(jī)制的理解方面發(fā)揮了關(guān)鍵作用。
我們現(xiàn)在提供96孔格式的冷凍保存和平板亞融合3T3-L1前脂肪細(xì)胞以及3T3-L1脂肪細(xì)胞增殖、分化和維持為脂肪細(xì)胞的培養(yǎng)基。我們還提供使用3T3-L1和/或初級(jí)嚙齒動(dòng)物前脂肪細(xì)胞和脂肪細(xì)胞驗(yàn)證的培養(yǎng)基和試劑盒。高質(zhì)量的對(duì)照測(cè)試了3T3-L1前脂肪細(xì)胞，其設(shè)計(jì)使用ZenBio的3T3-L1培養(yǎng)基系列持續(xù)工作。
“我們剛剛用從ZenBio獲得的3T3L1細(xì)胞進(jìn)行了一項(xiàng)實(shí)驗(yàn)，結(jié)果讓我們大吃一驚！在我們之前使用從ATCC和Sigma-Aldrich獲得的3T3T3L1的經(jīng)驗(yàn)中，獲得高百分比的細(xì)胞分化為成熟脂肪細(xì)胞一直是一項(xiàng)挑戰(zhàn)……我們獲得了幾乎100%的細(xì)胞向脂肪細(xì)胞的強(qiáng)烈分化。"第一次嘗試！"
Cryopreserved & Cultured 3T3-L1 Preadipocytes
| Item# | Item Desc | U/M |
|---|---|---|
| SP-L1-F | 3T3-L1 Preadipocytes Cryopreserved, (500,000 cells/vial) | Vial |
| SP-L1-96 | 3T3-L1 Preadipocytes 96-well Plate | Each |
| SP-L1-24 | 3T3-L1 Preadipocytes 24-well Plate | Plate |
| SP-L1-48 | 3T3-L1 Preadipocytes 48-well Plate | Each |
| SP-L1-T25 | 3T3-L1 Cultured Preadipocytes, T-25cm2 Flask | Flask |
Media
| Item# | Item Desc | U/M |
|---|---|---|
| AM-1-L1 | 3T3-L1 Adipocyte Medium | 500ml |
| AM-1-L1-PRF | 3T3-L1 Adipocyte Medium Phenol Red Free | 500ml |
| AM-1-L1-IF | 3T3-L1 Adipocyte Medium WITHOUT Insulin | 500ml |
| AM-1-L1-DF | 3T3-L1 Adipocyte Medium WITHOUT Dexamethasone | 500ml |
| AM-1-L1-SF | 3T3-L1 Adipocyte Medium WITHOUT Serum | 500ml |
| AM-1-L1-ABF | 3T3-L1 Adipocyte Medium WITHOUT ANTIBIOTICS | 500ml |
| DM-2-L1 | 3T3-L1 Differentiation Medium | 100ml |
| DM-2-L1-500 | 3T3-L1 Differentiation Medium | 500ml |
| DM-2-L1-PRF | 3T3-L1 Differentiation Medium, WITHOUT Phenol Red | 100ml |
| DM-2-L1-500-PRF | 3T3-L1 Differentiation Medium, WITHOUT Phenol Red | 500ml |
| DM-2-L1-ABF | 3T3-L1 Differentiation Medium, WITHOUT ANTIBIOTICS | 500ml |
| DM-2-L1-PPG | 3T3-L1 Differentiation Medium WITHOUT PPAR gamma agonist | 100ml |
| PM-1-L1 | 3T3-L1 Preadipocyte Medium | 500ml |
| PM-1-L1-PRF | 3T3-L1 Preadipocyte Medium Phenol Red Free | 500ml |
| PM-1-L1-ABF | 3T3-L1 Preadipocyte Medium, WITHOUT ANTIBIOTICS | 500ml |
| PM-1-L1-SF | 3T3-L1 Preadipocyte Medium, WITHOUT SERUM | 500ml |
| BM-1-L1 | 3T3-L1 Basal Medium | 500ml |
| FM-1-L1 | Cryopreservation Medium for 3T3-L1 cells | 100ml |
Kits
| Item# | Item Desc | U/M |
|---|---|---|
| LIP-1-L1 | Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, (REAGENTS+ CELLS) | Kit |
| LIP-1-L1-F | Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, WITH CRYOPRESERVED CELLS(REAGENTS+ CELLS) | Kit |
| LIP-1-NCL1 | Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, (REAGENTS ONLY) | Kit |
| LIP-1-NCL1DIF | Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection, WITH 3T3-L1 Media to differentiate 1 X 96 well plate (REAGENTS+ MEDIA) | Kit |
| KT-01 | 3T3-L1 Preadiopcyte Differentiation Kit Reagents sufficient to differentiate and maintain 1x96 well plate of 3T3-L1 Cells | Kit |
| KT-01-PRF | 3T3-L1 Preadipocyte Differentiation Kit: Reagents sufficient to differentiate and maintain 1x96 well plate of 3T3-L1 Cells (PHENOL RED- FREE) | Kit |
| LIP-2-NC-L1 | 3T3-L1 Lipolysis Assay Kit, Free Fatty Acid+ Detection,100 point assay kit (REAGENTS ONLY) | Kit |
| LIP-2-L1 | 3T3-L1 Lipolysis Assay Kit, Free Fatty Acid Detection (REAGENTS+ CELLS) | Kit |
| LIP-3-NC-L1 | 3T3-L1 Lipolysis Assay KIT (REAGENTS ONLY) Combo-Free Fatty acid and glycerol release | Kit |
| LIP-3-L1 | 3T3-L1 Lipolysis Kit- Dual Detection Free Fatty Acid and Glycerol Release 100 point assay kit,+ (REAGENTS CELLS) | Kit |
