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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>行業(yè)專用試劑>生物試劑>A00187 THE™ DYKDDDDK Tag Antibody, m...

A00187 THE™ DYKDDDDK Tag Antibody, mAb, Mouse

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漠寶(廈門)生物科技有限公司(Amobio Biotech Co.,Ltd)是一家致力于生命科學(xué)和生物技術(shù)領(lǐng)域的高科技企業(yè),依托于廈門大學(xué)人才力量。公司主要專注于細(xì)胞生物學(xué)與蛋白質(zhì)分析相關(guān)產(chǎn)品的開發(fā)與銷售。公司主要代理和自產(chǎn)國產(chǎn)優(yōu)質(zhì)試劑和設(shè)備,以期為生命科學(xué)多研究領(lǐng)域的科研人員提供美而精的產(chǎn)品。我們相信經(jīng)過幾年的努力,漠寶生物一定能成為細(xì)胞生物學(xué)、免疫學(xué)等領(lǐng)域優(yōu)質(zhì)的供應(yīng)商,促進(jìn)國產(chǎn)試劑替代進(jìn)口試劑的發(fā)展,加速國產(chǎn)關(guān)鍵性原料研發(fā)進(jìn)程,減輕困擾我們的進(jìn)口試劑和設(shè)備依賴病。


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供貨周期 一周 規(guī)格 100 μg
貨號 A00187 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,環(huán)保,食品/農(nóng)產(chǎn)品,生物產(chǎn)業(yè),制藥/生物制藥
主要用途 THE™ DYKDDDDK Tag Antibody, mAb, Mouse

產(chǎn)品概述

Specificity :THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.

Host Species: Mouse 

Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH 

Conjugate :Unconjugated 


應(yīng)用指導(dǎo)

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.  

Application:Recommended Usage

ELISA :0.05-0.2 µg/ml 

Western Blot: 0.1-1.0 µg/ml 

Immunoprecipitation (IP) :1 µg/ml 

Immunocytochemistry/Immunofluorescence (ICC/IF) :1 µg/ml 

Flow Cytometry: 1 µg/ml 


產(chǎn)品屬性

Form: Lyophilized 

Storage Buffer: Lyophilized with PB, pH 6.5, containing 0.02% sodium azide 

Reconstitution :Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

 Storage Instructions :The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. 

Purification :Protein A affinity column 

Isotype: Mouse IgG2b,κ 

Clonality: Monoclonal 

Clone ID :5A8E5 

Note :GenScript can offer this product per customer's request including product size, buffer components, etc.  


技術(shù)背景

Target Background: The DYKDDDDK peptide is a small epitope tag which is widely used in expression vectors. The tag does not appear to interfere with the bioactivity or bio-distribution of the tagged recombinant proteins. THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins

Synonyms: Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb 


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.




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