Mogengel Matrix

模基生物標準型金牌基質(zhì)膠是從富含胞外基質(zhì)蛋白的小鼠腫瘤中提取出的天然基底膜基質(zhì)。主要依次為層粘連蛋白（Laminin）、IV型膠原蛋白（Col-IV）、巢蛋白（Entactin）、硫酸乙酰肝素蛋白多糖（Heparan sulphate proteoglycans）及多種細胞因子，如類胰島素生長因子（IGF-1）、轉(zhuǎn)化生長因子β（TGF-β）、血管內(nèi)皮生長因子（VEGF）、表皮生長因子（EGF）、成纖維細胞生長因子（bFGF）等。產(chǎn)品溶解于高糖含酚紅DMEM中，且非定制產(chǎn)品均添加了50μg/mL慶大霉素。

細胞增殖或分化相關(guān)的二維或三維培養(yǎng)，以及細胞形態(tài)的研究，相關(guān)應(yīng)用主要有：細胞侵襲、血管生成和類器官培養(yǎng)等，?；锿扑]您在細胞遷移和侵襲、血管生成測定時選用標準型金牌基質(zhì)膠。

來源：小鼠腫瘤

外觀：①顏色：產(chǎn)品表現(xiàn)為黃色-粉紅色； ②形態(tài)：4℃融解后，呈液態(tài)

濃度：蛋白濃度范圍在8~12mg/mL之間

內(nèi)毒素：≤ 10EU/ mL

凝膠時間：室溫條件下5-30min凝膠，37°C時成膠速度加快

1、   根據(jù)GB 14922.2-2022檢測小鼠種群中以下病毒、病原菌寄生蟲及細菌結(jié)果為陰性。

2、   直接接種法檢測產(chǎn)品中是否含真菌、細菌，結(jié)果為陰性。

3、   對包括LDEV在內(nèi)的多種病原體進行廣泛的PCR檢測，確保對生產(chǎn)過程中使用的原材料進行嚴格控制。

4、   使用PCR技術(shù)擴增產(chǎn)品中支原體序列，結(jié)果為陰性。

5、   使用BCA方法測定蛋白濃度。

6、   使用凝膠限度檢查法檢測產(chǎn)品內(nèi)毒素水平。

7、   產(chǎn)品與培養(yǎng)基1:2比例稀釋后，置于37℃凝膠30分鐘，再加入培養(yǎng)基，產(chǎn)品能在37℃環(huán)境中保持這種形態(tài)5天。

8、   將產(chǎn)品稀釋至 70%含量，在細胞培養(yǎng)板中滴加50μL，37℃條件下凝膠30分鐘，可以形成穩(wěn)定的凝膠，加入培養(yǎng)基后，在37℃培養(yǎng)箱中能夠保持這種形態(tài)15天。

9、   每批次產(chǎn)品都能夠進行腫瘤細胞侵襲實驗和體外血管形成實驗。

a)   溫度控制

產(chǎn)品在≤-20℃時是穩(wěn)定的，分裝使用產(chǎn)品以盡可能減少產(chǎn)品的凍融次數(shù)。

請不要儲存在無霜冰箱中，長期保存時請務(wù)必保持產(chǎn)品的凍存狀態(tài)。

產(chǎn)品初次解凍時，請將西林瓶包埋在碎冰中，并放置在4℃冰箱中待其融解。

所有接觸產(chǎn)品的耗材，請?zhí)崆敖禍亍?/span>

請您在使用過程中不要過長時間地用手握住裝有本產(chǎn)品的容具，防止體溫使產(chǎn)品凝膠；若在較短時間內(nèi)造成產(chǎn)品較為厚重粘稠，您可以將本產(chǎn)品重新置于 0℃ - 4℃ 的環(huán)境內(nèi)1-2 h使其恢復(fù)流動性，不影響使用。

b)   避免污染

實驗操作人員需嚴格區(qū)分實驗操作臺、清潔區(qū)和污染區(qū)，確保插取吸頭、加樣、丟棄吸頭的動作呈單向流動。

c)   其他

產(chǎn)品在每次由冷凍狀態(tài)變?yōu)槿诮鉅顟B(tài)時，請適當搖晃或使用移液器吹吸，確保體系內(nèi)部蛋白分布均勻。

?；飿藴市徒鹋苹|(zhì)膠主要有四種使用方式，我們將為您提供這四種使用方式的的一般操作程序，您可以基于您的實驗?zāi)康倪x擇合適的使用方式。

V2.0版

更新時間：2025/6/12

    1、 m6A Modification of Long Non-Coding RNA HNF1A-AS1 Facilitates Cell Cycle Progression in Colorectal Cancer viaIGF2BP2-Mediated CCND1 mRNA Stabilization（IF=5.1 血管形成）

    2、 SIRT6PreventsGlucocorticoid-InducedOsteonecrosisofthe FemoralHeadinRats（IF=7.3 血管形成）

    3、 Polydopamine-coated thalidomide nanocrystals promote DSS-induced murine colitis recovery through Macrophage M2 polarization together with the synergistic anti-inflammatory and anti-angiogenic effects（IF=6.51  血管形成）

    4、 3D-printed Sr2ZnSi2O7 scaffold facilitates vascularized bone regeneration through macrophage immunomodulation（IF=6.006  血管形成）

    5、 Long noncoding RNA H19 synergizes with STAT1 to regulate SNX10 in rheumatoid arthritis（IF=4.17 侵襲實驗、血管生成）

    6、 Heteronuclear Ru(II)-Re(I) complexes as potential photodynamic anticancer agents with anti-metastatic and anti-angiogenic activities（IF=4.336 血管生成）

    7、 Efficient delivery of VEGF-A mRNA for promoting diabetic wound healing via ionizable lipid nanoparticles（IF=6.51 血管形成）

    8、 Flavokawain A suppresses the vasculogenic mimicry of HCC by inhibiting CXCL12 mediated EMT（IF=6.65 血管生成 ）

    9、 Recombinant Human Arresten and Canstatin Inhibit Angiogenic Behaviors of HUVECs via Inhibiting the PI3K/Akt Signaling Pathway（IF=6.2 血管生成）

    10、 H2S-releasing versatile hydrogel dressing with potent antimicrobial, anti-inflammatory, epithelialization and angiogenic capabilities for diabetic wound healing（IF=15.1 血管生成）

    11、 METTL3-mediated m6A RNA modification promotes corneal neovascularization by upregulating the canonical Wnt pathway during HSV-1 infection（IF=4.8 血管生成）

    12、 Down-regulation of histone deacetylase 7 reduces biological activities of retinal microvascular endothelial cells under high glucose condition and related mechanism（IF=1.4 血管生成）

    13、 Phenylboronic Ester-Bridged Chitosan/Myricetin Nanomicelle for Penetrating the Endothelial Barrier and Regulating Macrophage Polarization and Inflammation （IF=5.8   血管形成）

    14、 Efficient delivery of VEGFA mRNA for promoting wound healing via ionizable lipid nanoparticles（IF=3.5 血管形成）

    15、 Activation of angiotensin II type 2 receptor attenuates lung injury of collagen-induced arthritis by alleviating endothelial cell injury and promoting Ly6Clo monocyte …（IF=5   血管形成）

    16、  SIRT6 Prevents Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats（IF=7.3    血管形成）

    17、 Regulation of the Osteogenic Microenvironment by Pioglitazone-Loaded Polylactic Glycolic Acid Nanosphere Composite Scaffolds to Promote Vascularization and Bone Regeneration（IF=6.7  血管形成）

    18、 A Dose‐Dependent Spatiotemporal Response of Angiogenesis Elicited by Zn Biodegradation During The Initial Stage of Bone Regeneration（IF=10       血管形成）

    19、 MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy（IF=5.6 血管形成）

    20、 An injectable carrier for spatiotemporal and sequential release of therapeutic substances to treat myocardial infarction（IF=10.8 血管形成）

    21、 Sodium selenite ameliorates silver nanoparticles induced vascular endothelial cytotoxic injury by antioxidative properties and suppressing inflammation through activating the Nrf2 signaling pathway（IF=3.9 血管形成）

    22、 Hypoxic microenvironment reconstruction with synergistic biofunctional ions promotes diabetic wound healing（IF=10 血管形成）

    23、 Temperature-sensitive hydrogel dressing loaded with nicotinamide mononucleotide accelerating wound healing in diabetic mice（IF=7.5 血管形成）

    24、 Injectable photocuring silk fibroin-based hydrogel constructs antioxidant micro-environment for skin repair（IF=7 血管形成）

    25、 Temperature-sensitive hydrogel dressing loaded with nicotinamide mononucleotide accelerating wound healing in diabetic mice（IF=6.9  血管形成）

    26、 Discovery of novel amide derivatives against VEGFR-2/tubulin with potent antitumor and antiangiogenic activity（IF=4.5 血管形成）

    27、 Melatonin Attenuates Diabetic Retinopathy byRegulating EndMT of Retinal Vascular EndothelialCells via Inhibiting the HDAC7/FOXO1/ZEB1 Axis（IF=8.3  血管形成）

    28、 Biomimetic nanocomplex based cornealneovascularization theranostics Melatonin Attenuates Diabetic Retinopathy by Regulating EndMT of Retinal Vascular Endothelial Cells via Inhibiting the HDAC7/FOXO1/ZEB1 Axis（IF=10.5 血管形成）

    29、 Microenvironment Remodeling Self-Healing Hydrogel for Promoting Flap Survival（IF=8.1 血管形成實驗）

    30、 Multifunctional Exosomes Derived from M2 Macrophages with Enhanced Odontogenesis, Neurogenesis and Angiogenesis for Regenerative Endodontic Therapy: An In Vitro and In Vivo Investigation（IF=3.9 血管生成）

    31、 Multifunctional surface of the nano-morphic PEEK implant with enhanced angiogenic, osteogenic and antibacterial properties（IF=5.6 血管生成）

    32、 ABCA12 Promotes Proliferation and Migration and Inhibits Apoptosis of Pancreatic Cancer Cells Through the AKT Signaling Pathway ABCA12   （IF=4.77 侵襲實驗. ）

    33、 Progranulin promotes proliferation, migration, and invasion of eutopicendometrial stromal cells in patients with endometriosis via the PI3K/Akt signaling pathway Progranulin  （IF=4.5    侵襲實驗. ）

    34、 Exploration of fetal growth restriction induced by vitamin D deficiency in rats via Hippo-YAP signaling pathway（IF=3.28 侵襲實驗. ）

    35、 DEPDC1 as a crucial factor in the progression of human osteosarcoma DEPDC1  （IF=4.7  侵襲實驗）

    36、 TIAM2 Contributes to Osimertinib Resistance, Cell Motility,and Tumor-Associated Macrophage M2-like Polarization in Lung Adenocarcinoma（IF=6.2 侵襲實驗）

    37、 SUMOylation of TUFT1 is essential for gastric cancer progression through AKT/mTOR signaling pathway activation （IF=6.5 侵襲實驗）

    38、 Glycoside Combinations of BYHWD Inhibit Atherosclerotic Angiogenesis Via STAT3/HIF-1/VEGF Pathway BYHWD（IF=3.6 侵襲實驗）

    39、 "The Core-Targeted RRM2 Gene of Berberine HydrochloridePromotes Breast Cancer Cell Migration and Invasion via theEpithelial–Mesenchymal Transition（IF=5.21    侵襲實驗）

    40、  HOPX is a tumor-suppressive biomarker that corresponds to T cell infiltration in skin cutaneous melanoma（IF=5.8 侵襲實驗）

    41、 Elucidating the pharmacological effects of Compound Kushen injection on MYC-P15-CCND1 signaling pathway in nasopharyngeal carcinoma-An in vitro study（IF=5.4     侵襲實驗）

    42、 The Core-Targeted RRM2 Gene of Berberine Hydrochloride Promotes Breast Cancer Cell Migration and Invasion via the Epithelial–Mesenchymal Transition（IF=4.6 侵襲實驗）

    43、 Enhance Mitochondrial Damage by Nuclear Export Inhibition to Suppress Tumor Growth and Metastasis with Increased Antitumor Properties of Macrophages（IF=9.5       侵襲實驗）

    44、 M2 Macrophage-Derived Exosomal lncRNA MIR4435-2HG Promotes Progression of Infantile Hemangiomas by Targeting HNRNPA1（IF=8       侵襲實驗）

    45、 Nuciferine Inhibits Oral Squamous Cell Carcinoma Partially through Suppressing the STAT3 Signaling Pathway（IF=5.6 侵襲實驗）

    46、 Prognostic and immunological role of cancer-associated fibroblasts-derived exosomal protein in esophageal squamous cell carcinoma（IF=5.6     侵襲實驗）

    47、 TNFR2 promotes pancreatic cancer proliferation, migration, and invasion via the NF-κB signaling pathway（IF=5.2 侵襲實驗）

    48、 VAMP8 suppresses the metastasis via DDX5/β-catenin signal pathway in osteosarcoma（IF=3.6    侵襲實驗）

    49、 A novel necroptosis-related long noncoding RNA model for predicting clinical features, immune characteristics, and therapeutic response in clear cell renal cell carcinoma（IF=7.3     侵襲實驗）

    50、 6-Phosphogluconate dehydrogenase promotes glycolysis and fatty acid synthesis by inhibiting the AMPK pathway in lung adenocarcinoma cells（IF=9.1 transwell assay）

    51、 TREM1+ tumor-associated macrophages secrete CCL7 to promote hepatocellular carcinoma metastasis（IF=2.7   Wound-healing assay and transwell assay）