SK-NEP-1人腎母細(xì)胞瘤細(xì)
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人腎母細(xì)胞瘤細(xì)胞SK-NEP-1
Results: In melanoma cohorts, we found that ESTIMATE and immune scores were involved in survival or tumor clinical stage. Among the 22 immune cells, CD8+ T cells, M2 macrophages, and regulatory T cells (Tregs) showed significant differences using the CIBERSORTx algorithm. Furthermore, GSVA identified the immune cell-related pathways; the primary immunodeficiency pathway, intestinal immune network for IgA, and TGF-β pathways were identified as
| 種屬 | 人 |
| 別稱 | SKNEP-1; SKNEP1; SKNEP |
| 組織來(lái)源 | 腎 ，來(lái)源于轉(zhuǎn)移部位: 胸腔積液 |
| 疾病 | 尤因肉瘤 |
| 傳代比例/細(xì)胞消化 | 1:2傳代 |
| 培養(yǎng)基配置 | McCoy’s 5A培養(yǎng)基；10%胎牛血清；1%雙抗 |
| 簡(jiǎn)介 | 超微結(jié)構(gòu)有少許微絨毛 ，連接復(fù)合物 ，形態(tài)完整的高爾基體 ，內(nèi)質(zhì)網(wǎng)多為光滑型 ，脂滴 ，沒(méi)毒棵粒。 |
| 形態(tài) | 圓形 |
| 生長(zhǎng)特征 | 懸浮生長(zhǎng) |
| 倍增時(shí)間 | 每周 2 至 3 次 |
| 抗原表達(dá) | Blood Type A; Rh + |
| 致瘤性 | Yes, in nude mice; forms tumor with small cells consistent with Wilms' tumor. |
| STR | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,10 TH01: 8,9.3 TPOX: 8,11 vWA: 15,19 |
| 培養(yǎng)條件 | 氣相：空氣 ，95% ；二氧化碳 ，5%。 溫度：37攝氏度 ，培養(yǎng)箱濕度為70%-80%。 |
| 凍存條件 | 凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號(hào)JY-H040 |
| 保藏機(jī)構(gòu) | ATCC; HTB-48 |
| 備注 | 該細(xì)胞為懸浮細(xì)胞 ，請(qǐng)注意離心收集細(xì)胞懸液 ，請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。 |
Results: In melanoma cohorts, we found that ESTIMATE and immune scores were involved in survival or tumor clinical stage. Among the 22 immune cells, CD8+ T cells, M2 macrophages, and regulatory T cells (Tregs) showed significant differences using the CIBERSORTx algorithm. Furthermore, GSVA identified the immune cell-related pathways; the primary immunodeficiency pathway, intestinal immune network for IgA, and TGF-β pathways were identified as
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