小鼠宮頸癌細(xì)胞帶熒光素酶U14
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- 公司名稱 上海富雨生物科技有限公司
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小鼠宮頸癌細(xì)胞帶熒光素酶U14+LUC
stage disease, without evidence of lymph node or distant dissemination, relapse and die within 5 yr of curative resection. The inference is that occult or micrometastatic disease was already present at the time of resection, or that tumor cells with metastatic potential were released into the blood stream as a result of surgery. Detection of circulating tumor cells at diagnosis in early stage patients would imply that the disease is more advanced than indicated by conventional
| 種屬 | 小鼠 |
| 別稱 | U14+LUC |
| 組織來源 | 小鼠子宮頸 |
| 疾病 | 小鼠宮頸癌 |
| 傳代比例/細(xì)胞消化 | 1:2傳代,貼壁部分消化1-2分鐘,懸浮部分離心收集(1000rpm 離心 5min) |
| 培養(yǎng)基配置 | DMEM培養(yǎng)基；10%胎牛血清；1%雙抗 |
| 簡介 | 將U14瘤株腹腔接種于615小鼠 ，利用腹水進(jìn)行原代培養(yǎng) ，建立U14細(xì)胞系。615小鼠、C57BL/6小鼠移植成瘤率均 為99%。 |
| 形態(tài) | 上皮細(xì)胞樣 |
| 生長特征 | 懸浮生長 ，貼壁生長混合 |
| 倍增時(shí)間 | 每周 2 至 3 次 |
| 備注 | 該細(xì)胞為半懸浮和半貼壁細(xì)胞 ，懸浮細(xì)胞離心收集 ，貼壁細(xì)胞消化處理 ，該細(xì)胞是通過慢病毒轉(zhuǎn)染熒光素酶的穩(wěn)轉(zhuǎn) 株 ，收到細(xì)胞傳代8代左右后 ，若要求需要維持熒光強(qiáng)度 ，建議可以加入嘌呤霉素進(jìn)行再次篩選。 |
stage disease, without evidence of lymph node or distant dissemination, relapse and die within 5 yr of curative resection. The inference is that occult or micrometastatic disease was already present at the time of resection, or that tumor cells with metastatic potential were released into the blood stream as a result of surgery. Detection of circulating tumor cells at diagnosis in early stage patients would imply that the disease is more advanced than indicated by conventional
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