Product Description

The antibodies are developed from rabbit using affinity purified canine IgG as the immunogen. The canine IgG-specific antibodies from the rabbit immune serum are purified using canine IgG ligand affinity chromatography, followed by affinity absorption with human serum albumin, IgG from human, mouse, chicken and swine. The purified antibodies are linked to horse radish peroxidase (HRP) via reductive amination. These highly specific anti-canine IgG antibodies are useful for detection, identification, quantization, isolation and localization of canine IgG.

Species

Rabbit

Formulation

1 mg/mL in PBS, 50% glycerol

Immunogen

Affinity purified canine IgG

Purification

The antibody was affinity purified with canine antibodies immobilized on agarose.

Conjugation

Reductive amination with HRP/Ab ratio - 2:1

Specificity

The antibody is specific against canine IgG. It does not cross react with human, chicken, swine or mouse IgG.

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IHC; IP

Scientific Description

IgG is monomeric immunoglobulin. It is built of two heavy chains and two light chains. Each IgG has two antigen binding sites. It is the most abundant immunoglobulin in the blood and tissue. It provides protection against many kinds of bacteria, viruses, fungi as well as optimizes phagocytosis and neutralizes bacterial toxins.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

References

1. Junqueira, L. C. et al. Basic Histology, 2003 McGraw-Hill