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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標物>行業(yè)專用試劑>生物試劑>CRL-2149 SK-N-DZ細胞, 人成神經(jīng)瘤細胞-骨髓

CRL-2149 SK-N-DZ細胞, 人成神經(jīng)瘤細胞-骨髓

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  • 公司名稱 上海復祥生物科技有限公司
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  • 型號 CRL-2149
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  • 更新時間 2025/8/14 12:05:53
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上海復祥生物科技有限公司是一家專業(yè)從事原代細胞、細胞系;菌種;及細胞因子相關產(chǎn)品的銷售企業(yè)。公司建有自己的細胞庫和菌種庫。細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件,公司引進ATCC細胞500余種,ATCC菌種800余種。并和國內(nèi)各大保藏中心有密切聯(lián)系,細胞種類齊全,質(zhì)量保證!是生命科學領域*的一部分!

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ATCC 細胞,細胞系,細胞株,腫瘤細胞,細胞,ATCC 菌種,CMCC 菌種,標準菌株,質(zhì)控菌種,微生物菌種,菌株,菌種

供貨周期 一周 規(guī)格 T25
貨號 SK-N-DZ 應用領域 醫(yī)療衛(wèi)生,食品/農(nóng)產(chǎn)品,化工,生物產(chǎn)業(yè),制藥/生物制藥
主要用途 科學研究

SK-N-DZ細胞, 人成神經(jīng)瘤細胞-骨髓

ATCC® Number:CRL-2149™    Price:$338.00
Designations:SK-N-DZDepositors:C HelsonBiosafety Level:1Shim & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)pped:frozenMediuMorphology:epithelial Source:Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow Cell Type: neuroblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.SK-N-DZ細胞, 人成神經(jīng)瘤細胞-骨髓Tumorigenic:YesDNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 9,11 D5S818: 12 D7S820: 12,13 THO1: 6,9 TPOX: 8 vWA: 16,18Cytogenetic Analysis:modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seenAge:2 yearsGender:femaleEthnicity:CaucasianComments:SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma. Retinoic acid induces differentiation in this line. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression.Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 661079222439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 841782423127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694Related Links NCBI Entrez SearchMake a DepositFrequently Asked QuestionsMaterial Transfer AgreementTechnical SupportRelated Cell Culture Products

ATCC® Number: CRL-2149™ Price: $338.00
Designations: SK-N-DZ
Depositors: C Helson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial


Source: Organ: brain
Disease: neuroblastoma
Derived from metastatic site: bone marrow
Cell Type: neuroblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18
Cytogenetic Analysis: modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen
Age: 2 years
Gender: female
Ethnicity: Caucasian
Comments: SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.
Retinoic acid induces differentiation in this line.
Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.
Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.
The cells exhibit moderate MDR1 expression.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792
22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824
23127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694
Related Links
NCBI Entrez Search
Make a Deposit
Frequently Asked Questions
Material Transfer Agreement
Technical Support
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